mouse fcgriv  (BPS Bioscience)

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Trastuzumab triggers phagocytic killing of high HER2 cancer cells in vitro and in vivo by interaction with Fcγ receptors on macrophages.

doi: 10.4049/jimmunol.1402891

Figure Lengend Snippet: FIGURE 4. FcgRIV expression is associated with trastuzumab anticancer efficacy. (A) FcgRIV shows stronger binding affinity to trastuzumab in comparison with other mouse FcgRs. The x-axis is at log scale and data points are average of three replicates by ELISA. Error bars indicate the SDs. (B) Higher FcgRIVexpression was detected in tumor-infiltrated immune cells treated with trastuzumab than that of the isotype control by immunofluorescence. OCT frozen tumor tissues were stained with a rabbit anti-FcgRIV Ab and detected with an Alexa Fluor 488–anti-rabbit IgG Ab. A representative image is shown. Original magnification 340. (C) FcgRIV expression on different types of macrophages by flow cytometry using the same Ab set as in (B). MFI is shown in the histogram.

Article Snippet: Measurement of FcgR expression and FcgRs binding assays Macrophages were stained with rabbit anti-mouse FcgRIV (Creative Biomart, Shirley, NY) and followed by Alexa Fluor 488–anti-rabbit IgG (Life Technologies).

Techniques: Expressing, Binding Assay, Comparison, Enzyme-linked Immunosorbent Assay, Control, Staining, Cytometry

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Trastuzumab triggers phagocytic killing of high HER2 cancer cells in vitro and in vivo by interaction with Fcγ receptors on macrophages.

doi: 10.4049/jimmunol.1402891

Figure Lengend Snippet: FIGURE 5. FcgRIV expression on macrophage cells is associated with ADCP and cancer killing mediated by trastuzumab. (A) FcgRIV expression was detected by flow cytometry in WT, FcgRIV KD, and WT plus IFN-g macrophage cells. MFI as shown in respective histograms. (B) Cancer cell killing by WT, FcgRIV KD, and WT plus IFN-g macrophage cells in the presence of trastuzumab. The percentage of cell killing is calculated using the cell index collected by the xCELLigence instrument after 24 h of trastuzumab treatment. (C) ADCP activity of WT, FcgRIV KD, and WT plus IFN-g macrophage cells in the presence of trastuzumab. The percentage of phagocytosis was calculated from the double-positive cells by flow cytometry as described in Fig. 2A. The results are shown as mean 6 SD; n = 3. *p , 0.05. (D) FcgR IVexpression in BMMs with or without IFN-g (50 ng/ml) stimulation for 24 h by flow cytometry. (E) Cancer cell lysis mediated by BMMs or IFN-g–stimulated BMMs in the presence of trastuzumab. (F) ADCP activity of BMMs and IFN-g–stimulated BMMs mediated by trastuzumab as determined by flow cytometry. Percentage of phagocytosis was calculated as described in Fig. 2A. All experiments were repeated three times and error bars in the graphs show the SD. *p , 0.05.

Article Snippet: Measurement of FcgR expression and FcgRs binding assays Macrophages were stained with rabbit anti-mouse FcgRIV (Creative Biomart, Shirley, NY) and followed by Alexa Fluor 488–anti-rabbit IgG (Life Technologies).

Techniques: Expressing, Cytometry, Activity Assay, Lysis